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This Concept Map, created with IHMC CmapTools, has information related to: Avian influenza VF, If suspicion confirmed: dir 2005/94 Dir 2005/94 Protection zone: min. 3 km, Guarantee healthy animals before importation via veterinary certificate ???? General legislation, Avian Influenza Diagnostic assays Litterature review, Virology OIE OIE prescribed assays, Virology Wild birds from sensitive species: 3500/year* 500 swabs from hunted birds (ULg) PCR (Influenza A) Samples:faeces, large/small intestine, trachea, lung, oropharyngeal/tracheal/cloacal swabs Pooled samples: yes, 5 swabs or 5 organs maximum Species: poultry, pigeons, wild birds Se.: 4.57 EID50/ml Sp.:ND Detection: from 1 dpi (HPAI), 2-3 (LPAI) dpi to 10-15 dpi Samples/day:200 organs-400 swabs (r)/ (c) Results (days): 6 to ᡄ hours (but depends on the number of samples)(r)/(c) 3 days to swith the flow fro r to c Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample:52.3 €, Avian Influenza Diagnostic assays Commercial kits, Freedom Individual certification for exportation CODA-CERVA, CODA-CERVA 10 chickens or 50 ducks/farm* cELISA, CODA-CERVA Resp. B.Bénédicte Lambrecht Tel: +32 (0)2/ 379 05 59 Passive surveillance (2010/367) Symptomatic and dead wild and domestic birds from sensitive species, PCR (influenza A) 10 samples/focal spot If positif first samples H.I., Avian Influenza Scenario Spread, PCR (Influenza A) Samples:faeces, large/small intestine, trachea, lung, oropharyngeal/tracheal/cloacal swabs Pooled samples: yes, 5 swabs or 5 organs maximum Species: poultry, pigeons, wild birds Se.: 4.57 EID50/ml Sp.:ND Detection: from 1 dpi (HPAI), 2-3 (LPAI) dpi to 10-15 dpi Samples/day:200 organs-400 swabs (r)/ (c) Results (days): 6 to ᡄ hours (but depends on the number of samples)(r)/(c) 3 days to swith the flow fro r to c Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample:52.3 € If positif first samples Sequencing (ABI 3130) Samples/day: 38 Results: 2.5 hours (only the sequencing) Guard duty:no Accreditation (17025): no, Early detection Surveillance OIE recommandations Chap.10.4.27 to 10.4.29, Symptomatic and dead wild and domestic birds from sensitive species Suspicion PCR (Influenza A) Samples:faeces, large/small intestine, trachea, lung, oropharyngeal/tracheal/cloacal swabs Pooled samples: yes, 5 swabs or 5 organs maximum Species: poultry, pigeons, wild birds Se.: 4.57 EID50/ml Sp.:ND Detection: from 1 dpi (HPAI), 2-3 (LPAI) dpi to 10-15 dpi Samples/day:200 organs-400 swabs (r)/ (c) Results (days): 6 to ᡄ hours (but depends on the number of samples)(r)/(c) 3 days to swith the flow fro r to c Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample:52.3 €, Importation Live animals Specific legislation, Avian Influenza Scenario Early detection, CODA-CERVA Resp. B.Bénédicte Lambrecht Tel: +32 (0)2/ 379 05 59 Active surveilance (2010/367)* Number of holdings and birds to be sampled, Symptomatic and dead wild and domestic birds from sensitive species Suspicion Prescribed assays, Spread OIE Prescribed assays, Specific legislation ???? RE 798/2008