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This Concept Map, created with IHMC CmapTools, has information related to: Swine vesicular disease VF, Duplex PCR Samples: idem V.I. Pooled samples: Species: pigs Se./Sp.: > 95% Detection:blood: 1-2 days before the lesions appear During the period lesions are present Samples/day: 6-10/person (ur.), 15 (c) 3 days to switch the flow Results (days): 8 opened hours (ur), 1 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 80.4-96.5€ confirmation Antigen ELISA Samples: idem V.I. Pooled samples: Species: pigs Se.: medium Sp.: > 95% Detection:blood: 1-2 days before the lesions appear During the period lesions are present Samples/day: 6-10/person (ur.), 15 (c) 5 days to switch the flow Results (days): 8 hours (ur.),15 (r), 2 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample:31.8-50.2€, If positif Take blood samples In the positif pig(s)+ pigs in the same holding in order to detect 50% prev with 95% C.I., OIE prescribed assays ???? Competitive ELISA, DuplexPCR Competitive ELISA 2nd year cELISA, Freedom Repopulation > 21 days after completion of the operations, If serologic surveillance in importating holdings. Number of samples Enough to detect 5% prev with 95% C.I., Clinical suspicion CODA-CERVA Resp. K. De Klercq K. Tel : +32 (0)2/ 379 05 Duplex PCR Samples: idem V.I. Pooled samples: Species: pigs Se./Sp.: > 95% Detection:blood: 1-2 days before the lesions appear During the period lesions are present Samples/day: 6-10/person (ur.), 15 (c) 3 days to switch the flow Results (days): 8 opened hours (ur), 1 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 80.4-96.5€, Swine vesicular disease Scenarios Importation, E.U. specific legislation ???? If serologic surveillance in importating holdings., Swine vesicular disease Scenarios Early detection, V.I. Samples: tonsil(post mortem), uncoagulated blood, coagulated blood, muccocutaneous lesions, vesicle fluid, epithelium covering unruptured vesicles, epithelial flaps of freshly ruptured vesicles, or swabs of the freshly ruptured vesicles (oropharyngeal swabs, nasal swabs), faeces Pooled samples: Species: pigs Se./Sp.: > 95% Detection:blood: 1-2 days before the lesions appear During the period lesions are present Samples/day: 6-10/person (ur.), 15 (c) 5 days to switch the flow Results (days):3 (ur.),16 (r), 2(c) Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample:68.1- 81.7€ confirmation Antigen ELISA Samples: idem V.I. Pooled samples: Species: pigs Se.: medium Sp.: > 95% Detection:blood: 1-2 days before the lesions appear During the period lesions are present Samples/day: 6-10/person (ur.), 15 (c) 5 days to switch the flow Results (days): 8 hours (ur.),15 (r), 2 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample:31.8-50.2€, Importation Live animal E.U. specific legislation, V.I. Samples: tonsil(post mortem), uncoagulated blood, coagulated blood, muccocutaneous lesions, vesicle fluid, epithelium covering unruptured vesicles, epithelial flaps of freshly ruptured vesicles, or swabs of the freshly ruptured vesicles (oropharyngeal swabs, nasal swabs), faeces Pooled samples: Species: pigs Se./Sp.: > 95% Detection:blood: 1-2 days before the lesions appear During the period lesions are present Samples/day: 6-10/person (ur.), 15 (c) 5 days to switch the flow Results (days):3 (ur.),16 (r), 2(c) Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample:68.1- 81.7€ confirmation PCR, OIE prescribed assays ???? Competitive ELISA, Differential diagnosis with FMD (5-6 pigs are sampled) based on Demonstration of a specific antibody response in serum samples, OIE prescribed assays ???? Complement fixation, Differential diagnosis with FMD (5-6 pigs are sampled) based on Detection of clinical signs of disease, Swine vesicular disease Scenarios Spread, V.I. Samples: tonsil(post mortem), uncoagulated blood, coagulated blood, muccocutaneous lesions, vesicle fluid, epithelium covering unruptured vesicles, epithelial flaps of freshly ruptured vesicles, or swabs of the freshly ruptured vesicles (oropharyngeal swabs, nasal swabs), faeces Pooled samples: Species: pigs Se./Sp.: > 95% Detection:blood: 1-2 days before the lesions appear During the period lesions are present Samples/day: 6-10/person (ur.), 15 (c) 5 days to switch the flow Results (days):3 (ur.),16 (r), 2(c) Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample:68.1- 81.7€ confirmation Virus neutralization test Samples: Uncoagulated blood (serum) Pooled samples: Species: pigs Se./Sp.: > 95% DIVA: no Detection: 7 dpi to dpi Samples/day: 5(r), 10 (c.) 3 days to switch the flow Results (days): 3 (ur./c), 9 (r) Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): yes Price/sample:68.1-81.7€, Importation Live animal Guarantee healthy animals before importation via veterinary certificate