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This Concept Map, created with IHMC CmapTools, has information related to: Bluetongue VF, Protection zone:min.100 km which include Vaccination zone, can be Active surveillance DGZ Resp. M. Vanrobaeys Tel 0473 222547, Re 1266/2007 To demonstrate the absence during min 2 years of certain ser. 5% prev. with 95% confidence Theorical sample size: 398 animals 1X/year (worst case), PCR ADIAVET™ BTV REAL TIME Biomérieux Samples: uncoagulated blood, tissues Pooled samples: Species: ruminants Se:100%, LoD is comprised under 5 to 10 copies/assay Sp:100% Serotyping: no (all serotypes) Detection: from 10 dpi to 30 dpi Samples/day:10(r)/30 (c) Results (days): 5 (r), 3 (c) 14 days to swith the flow Experience in diagnotic: yes Directly functional: yes Guard duty:yes Accreditation (ISO 17025): yes Price/samples::41-60 € confirmation PCR (in house) Samples: uncoagulated blood, spleen, brain, lymph nodes pooled samples: Species: ruminants, insects Se.: 100% LOD 141.1 copies RNA/ reaction Sp.: 100% Serotyping: no Detection: from 2 to 4 dpi to ᡍ dpi Samples/day:3 hours/ 20 samples (r)/ (c) Results (days): 8 hours (ur.), 8 (uncoagulated blood) 14 (others),1 (c) 1 day to swith the flow Experience in diagnotic: yes Guard duty: no Directly functional: yes Accreditation (ISO 17025): yes Price/samples:30.5-38€, Spread CODA-CERVA PCR, Early detection Surveillance OIE assays prescribed, can be Active surveillance Re 1266/2007, Bluetongue Diagnostic assays Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Re 1266/2007 To detect any possible incursions, 1X/year min 20% prev. with 95% conf. 39 animals (worst case), In Belgium: 1702 cows in 2015 "winter screening" Competitive ELISA IDEXX Bluetongue Competition Ab Test- IDEXX Samples: Uncoagulated blood Pooled samples: no Species: ruminants Se:90-100% Sp:100% Serotyping: no (all serotypes) DIVA: no Detection: from 21 dpi to dpi Samples/day:100(r)/1400 (c) Results (days): 7 (r), 3 (c) 21 days to swith the flow Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/samples:6.84 €, E.U. legislation and Animals had remained since birth or min 40 days in a area (min 10 km radius) there has been no case of BTV for 40 days, Importation Live animals OIE recommendations for importations Chap. 8.3.6 to 8.3.8, Bluetongue Scenarios Importation, Suspicion Definition RE 1266/2007 Art. 2, Suspicion ???? OIE prescribed assays, Protection zone:min.100 km which include Vaccination zone, OIE recommendations for importations Chap. 8.3.6 to 8.3.8 and Prescribed assays, Early detection Surveillance can be, can be Active surveillance ARSIA Resp. G.Maquet Tel 083 23 05 15, If suspicion confirmed Dir 2000/75 Dir 92/119 Surveillance zone:min. 50km