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This Concept Map, created with IHMC CmapTools, has information related to: Newcastle Disease VF, Importation Live animals Specific legislation, PCR Samples: faeces, large/small intestine, trachea, lung oropharyngial/tracheal/faecal/cloacal swabs Pooled samples: yes, max. 5 swabs Species: pigeons, poultry Limit of detection: 251 EID50/ml Sp.: ND Serotyping: yes, PMV-1 Detection: from 3 dpi to 10-15 dpi Samples/day: ND (r), 200 organs – 400 swabs max (c) Results(days): 6 hours (ur.), 1(c) 3 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample: 68.2€-95€ If + confirmation first samples V.I. Samples: faeces, large/small intestine, trachea, lung oropharyngial/tracheal/faecal/cloacal swabs Pooled samples: Species: pigeons, poultry Se./Sp.: gold standard Detection: from 5-7 dpi Samples/day: 10(r,c) Results (days): 2 to 10 Experience in diagnotic: yes Guard duty: yes Directly functional: yes Accreditation (ISO 17025): yes Price/sample: 68.2 -110 €, CODA-CERVA ???? Haemagglutination inhibition Samples: serum Pooled samples: Species: pigeons, poultry Se./Sp.: gold standard Serotyping: yes, PMV1 to PMV-9 DIVA: no Detection: from 5 to 7 Samples/day: 400 (r) Results (days): 8 hours (ur.) Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample: 3.3-8.1€, PCR Samples: faeces, large/small intestine, trachea, lung oropharyngial/tracheal/faecal/cloacal swabs Pooled samples: yes, max. 5 swabs Species: pigeons, poultry Limit of detection: 251 EID50/ml Sp.: ND Serotyping: yes, PMV-1 Detection: from 3 dpi to 10-15 dpi Samples/day: ND (r), 200 organs – 400 swabs max (c) Results(days): 6 hours (ur.), 1(c) 3 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample: 68.2€-95€ If + confirmation first samples Sequencing (ABI 3130) Samples/day: 38 Results: 2.5 hours (only the sequencing) Guard duty:no Accreditation (17025): no, Importation Live animals OIE recommandations Art. 10.9.4 to 10.9.7, Newcastle Disease Scenarios Spread*, Importation Live animals Guarantee healthy animals before importation via veterinary certificate, E.U. legislation concerning vaccination RE 955/2010 93/152/CEE, Newcastle Disease Scenarios Early detection, Clinical suspicion CODA-CERVA Resp. B. Lambrecht Tel:+32 (0)2/ 379 05 59 PCR Samples: faeces, large/small intestine, trachea, lung oropharyngial/tracheal/faecal/cloacal swabs Pooled samples: yes, max. 5 swabs Species: pigeons, poultry Limit of detection: 251 EID50/ml Sp.: ND Serotyping: yes, PMV-1 Detection: from 3 dpi to 10-15 dpi Samples/day: ND (r), 200 organs – 400 swabs max (c) Results(days): 6 hours (ur.), 1(c) 3 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample: 68.2€-95€, Newcastle Disease Scenarios Freedom, CODA-CERVA ???? PCR, Specific legislation ???? RE 798/2008, Newcastle Disease Scenarios Importation, Newcastle Disease Diagnostic assays Commercial kits, Spread* CODA-CERVA PCR, PCR If + first samples V.I., Guarantee healthy animals before importation via veterinary certificate ???? General legislation, If suspicion confirmed Dir 92/66 Protection zone: min 3 km, Freedom Free country or zone CODA-CERVA