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This Concept Map, created with IHMC CmapTools, has information related to: African Swine Fever VF, > 40 days after completion of the cleaning, disinfection, disinsectisation operations Open-air holdings Sentinel pigs which are sampled 45 days after introduction, Freedom Individual certification for exportation OIE prescribed assays for international trade, Freedom Individual certification for exportation CODA-CERVA, Clinical suspicion CODA-CERVA Tel:0479 81 92 93 0471 34 87 32 PCR Samples: idem V.I. Pooled samples: no Species: pigs, boars Se.:ᢒ% Sp.:ᢒ% Detection: from 3 dpi to 45 dpi Samples/day: 40 (ur.), 400 (c) Results (day): 5 hours (ur.), 1 (c), 6 (r) 1 day to swith the flow Experience in diagnotic: yes Directly functional: yes Guard duty:no Accreditation (ISO 17025): no Price/sample:37.1€-53.4€, Blocking ELISA Ingezim PPA compact - Ingenasa Samples: coagulated blood (serum) Pooled samples: no Species: pigs, boars Se.: 97.5% Sp.: 99.5% Diva: no Detection: from 10 dpi to dpi Samples/day:40 (r),250-400 (c) Results (days): 4 hours (ur.), 6 (r), 1 (c) 3 days to swith the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample:21.3€ If first positive first samples V.I.-hemadsorption Samples:uncoagulated blood, coagulated blood, lymph node, spleen, kidney, tonsil Pooled samples: no Species: pigs, boars Se: ND Sp: > 90% Detection: from 4 dpi to 30 dpi Samples/day:2(r),15-20(c) Results in 1 month(r/c) 1 day to swith the flow Experience in diagnotic: yes Directly functional: yes Guard duty:no Accreditation (ISO 17025): no Price/sample:68.9 €, Antigen ELISA Alternative IFA, Spread CODA-CERVA PCR, If suspicion confirmed dir. 2002/60 Protection zone: min.3 km, African Swine Fever Scenario Early detection, Indirect ELISA Alternative IFA, Sentinel pigs which are sampled 45 days after introduction Serologic tests to detect 10% seroprev. and 95% C.I. If no antibody >< ASF detected OK for repopulation, Protection zone: min.3 km during min 30 days(dir 82/320), Importation Live animals OIE recommandations for importations Chap. 15.1.5 to 15.1.7, Guarantee healthy animals before importation via veterinary certificate ???? General legislation, OIE prescribed assays for international trade ???? Antigen ELISA, OIE prescribed assays for international trade ???? Indirect ELISA, Early detection Passive surveillance Clinical suspicion, Blocking ELISA Ingezim PPA compact - Ingenasa Samples: coagulated blood (serum) Pooled samples: no Species: pigs, boars Se.: 97.5% Sp.: 99.5% Diva: no Detection: from 10 dpi to dpi Samples/day:40 (r),250-400 (c) Results (days): 4 hours (ur.), 6 (r), 1 (c) 3 days to swith the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample:21.3€ If first positive first samples V.I.-hemadsorption UE-RL CISA-INIA, Madrid Samples:uncoagulated blood, coagulated blood, lymph node, spleen, kidney, tonsil Pooled samples: Samples/day: 20 Results in 1 month after reception Guard duty: no Accreditation (ISO 17025): no, African Swine Fever Diagnostic assays Commercial kits, Freedom Free country or zone OIE definition freedom from infection Chap. 15.1.2 to 15.1.3