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This Concept Map, created with IHMC CmapTools, has information related to: Easter western enceph and Venezu enceph VF, Passive surveilance Clinical suspicion 3 specific PCR Samples: uncoagulated blood Pooled samples: no Species:all species Se./Sp.: > 90% Detection: from 2 to 4 dpi Samples/day: 72(ur.), 400(c) Results (days): 2 to 5 (ur.), 1 (c) 1 day to switch the flow Experience in diagnotic: no Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 10.9€-29.8€ (EEV,VEEV), on request (WEEV), Eastern/Western/Venezuelian encephalitis Scenario Spread, Freedom Free country or zone OIE definition VEEV: chap 12.11.2, Importation Live animals Guarantee healthy animals before importation via veterinary certificate, Passive surveilance Clinical suspicion OIE prescribed assays, Early detection Surveillance OIE recommandations, Importation Live animals OIE recommandations and precribed assays EEEV, WEEV: art 12.2.4 VEEV: art 12.11.23 to 12.11.5, Early detection Surveillance Passive surveilance, PCR Samples: whole blood Pooled samples: no Species: horses Se./Sp.: ᢗ% Samples/day: 50 (r,c) Results (days): 7 (r),1 (c) after reception Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no If positive V.I Samples:brain Results (days): 21 Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no, Passive surveilance Clinical suspicion Official confirmation, Eastern/Western/Venezuelian encephalitis Scenario Importation, Eastern/Western/Venezuelian encephalitis Scenario Freedom, Eastern/Western/Venezuelian encephalitis Scenario Early detection, Freedom ANSES Virus neutralisation test, Eastern/Western/Venezuelian encephalitis Diagnostic assays Literature review, Official confirmation ANSES Sylvie Lecolinet Tel: +33 (0)1 49 77 13 50 PCR Samples: whole blood Pooled samples: no Species: horses Se./Sp.: ᢗ% Samples/day: 50 (r,c) Results (days): 7 (r),1 (c) after reception Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no, 3 specific PCR Samples: uncoagulated blood Pooled samples: no Species:all species Se./Sp.: > 90% Detection: from 2 to 4 dpi Samples/day: 72(ur.), 400(c) Results (days): 2 to 5 (ur.), 1 (c) 1 day to switch the flow Experience in diagnotic: no Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 10.9€-29.8€ (EEV,VEEV), on request (WEEV) confirmation Sequencing (ABI 3130) Samples/day: 38 Results: 2.5 hours (only the sequencing)-> 2-3 days entire process Guard duty:no Accreditation (17025): no, Spread CODA-CERVA PCR, Eastern/Western/Venezuelian encephalitis Diagnostic assays OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Official confirmation ANSES Sylvie Lecolinet Tel: +33 (0)1 49 77 13 50 VNT (differenciate WNV,JEV,EEEV,WEEV,VEEV) Samples: serum Pooled samples: no Species: horses DIVA: no Detection: from 10-15 dpi Samples/day: 50 (r,c) Results (days): 10(r), 7(c) after reception Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): no