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This Concept Map, created with IHMC CmapTools, has information related to: Foot and mouth disease VF, Freedom Repopulation Min. 21 days after disinfection (Dir. 2003/85, annexe V), Surveillance Passive surveillance Clinical suspicion, Surveillance ???? OIE principes and methods for surveillance Art. 8.8.40 to 8.8.41, Had remained since birth or min 40 days in a area (min 10 km radius) there has been no case of FMD for 40 days If requested Oesophageal/pharyngeal samples for V.I., CODA-CERVA 1st year Duplex PCR Competitive ELISA, Surveillance zone:min. 10 km ???? Duration, Foot and mouth disease Diagnostic assays Commercial kits, Freedom Free country or zone CODA-CERVA, Guarantee healthy animals before importation via veterinary certificate ???? General legislation, Clinical suspicion CODA-CERVA Resp. K. De Klercq K. Tel : +32 (0)2/ 379 05 Competitive ELISA (discriminate between the 7 serotypes) Samples: coagulated blood Pooled samples: no Species: cattle, pigs, small ruminants Se.: > 95% Sp.:ᢗ% Serotyping: yes DIVA: no Detection: from 4 dpi until 180 dpi Samples/day: 10 (r/c) Results (days): 8 hours (ur.), 7 (r), 1 (c) 3 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes (A,O,Asia) Price/sample: 21.3-25.5 €, Duplex PCR Samples:idem V.I. Pooled samples: no Species: cattle, pigs, small ruminants Se./Sp.: > 99% Serotyping: no Detection: blood: 1-2 days before the lesions appear. During the period lesions are present Samples/day: 6/person (ur.) Results (days): 8 opened hours (ur),6 (r), 1 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): yes Price/sample: 80.4-96.5 € confirmation Sequencing (ABI 3130) Samples/day: 38 Results: 2.5 hours (only the sequencing) Guard duty:no Accreditation (17025): no, Early detection ???? Surveillance, Suspicion without clinical sign Take enough samples to detet 5% prev. wirth 95% C.I. (Dir. 2003/85) Epi tools, PCR confirmation (if requested) PCR, V.I. Samples:uncoagulated blood, coagulated blood, muccocutaneous lesions, unruptured vesicules, oropharyngeal swabs, nasal swabs, swabs from freshly ruptured vesicles, saliva Pooled samples: no Species: cattle, pigs, small ruminants Se.: > 95% Sp.:ᢗ% Serotyping: no Detection: not assessed Samples/day: 6-10 (ur.), 15 (c) Results (days): 3 (if +), 16 (if -) 3 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample: 24.2-81.7 € confirmation PCR, Specific E.U. legislation animals Had remained since birth or min 40 days in a area (min 10 km radius) there has been no case of FMD for 40 days, V.I. Samples:uncoagulated blood, coagulated blood, muccocutaneous lesions, unruptured vesicules, oropharyngeal swabs, nasal swabs, swabs from freshly ruptured vesicles, saliva Pooled samples: no Species: cattle, pigs, small ruminants Se.: > 95% Sp.:ᢗ% Serotyping: no Detection: not assessed Samples/day: 6-10 (ur.), 15 (c) Results (days): 3 (if +), 16 (if -) 3 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: yes Accreditation (ISO 17025): yes Price/sample: 24.2-81.7 € confirmation Sequencing (ABI 3130) Samples/day: 38 Results: 2.5 hours (only the sequencing) Guard duty:no Accreditation (17025): no, Foot and mouth disease Diagnostic assays OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Importation Live animals OIE recommandations Chap. 8.8.10 to 8.8.12, Protection zone:min. 3 km Includes Suppressive vaccination